Opsonic effect of equine plasma from different donors
Veterinary Microbiology 56 (1997) pages 227-235
reprinted with permission of Elsevier Science
Gittan Grondahl, Anders Johannisson, Marianne Jense-Waern
Abstract
The ability of equine plasma from different donors to enhance phagocytic capacity was assessed in neutrophils obtained from seven foals, aged 7-8 days (Study A), and from seven adult horses (Study B). Neutrophils were allowed to phagocytize fluorescent yeast cells opsonized with plasma from one of three donors or with pooled serum, all previously frozen (- 18 C) and thawed. The results were analyzed by flow cytomentry. In study A, fresh autologous foal serum was also used for oponization, and in study B, heat-inactivated plasma and pooled serum were used in addition to untreated samples. The plasma from donor GN induced a higher number of truly phagocytic neutrophis. (mean 78%) than did plasma from donors GD (68%), OD (66%) and pooled serum (59&) when neutrophils were used. Phagacytosis was markedly reduced with with heat-inactivated plasma as a result of there being pure phagocytic neutrophils and less phagocytized material per cell. The opsonic capacities of the autologous foal sera were lower than that of adult donor plasma in six out of seven foals. It is concluded that there is significant individual variation in the opsonic activity amongst plasma donors with similar serum IgG concentrations. The results were consistent irrespective of whether neutrophils from adults or foals were used. @1997 Elsevier Science B.V.
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